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3d montages  (Oxford Instruments)


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    Oxford Instruments 3d montages
    3d Montages, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 43936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+montage/pm36541039-238-16-23?v=Oxford+Instruments
    Average 99 stars, based on 43936 article reviews
    3d montages - by Bioz Stars, 2026-08
    99/100 stars

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    3d montages  (Oxford Instruments)
    99
    Oxford Instruments 3d montages
    3d Montages, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+montage/pm36541039-238-16-23?v=Oxford+Instruments
    Average 99 stars, based on 1 article reviews
    3d montages - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    3d montage  (Oxford Instruments)
    99
    Oxford Instruments 3d montage
    ERα is present at higher concentrations in female than in male CA1 synapses. A, Top <t>left,</t> <t>Deconvolved</t> two-photon microscopic images of immunofluorescent labeling were used to construct a <t>3D</t> montage of the CA1 sample field (shown); one can see that a subpopulation of PSD-95-IR contacts (green) also contains ERα immunoreactivity (double labeling appears yellow). Scale bar, 2 μm. Top right, Image of a single double-labeled PSD shows the spatial relationship of areas occupied by PSD-95 (green) and ERα (red) immunoreactivities and the extent of overlap (merge, yellow). Scale bar, 0.1 μm. Bottom, Image shows the montage from the same z-stack illustrated in the top left but with the top of that panel rotated away from the viewer to show double labeling of the same puncta (arrows) from a different 3D viewpoint. B, The density frequency distribution for ERα-IR (colocalized with PSD-95) shows a greater rightward skew in females relative to males (p < 0.0001, F(19,646) = 17.28; males, n = 12; female, n = 24). C, Bar graph shows the percentage of double-labeled synapses with high concentrations of ERα immunolabeling (density units of ≥90) normalized to the mean male value shows that there were far more dense ERα-IR synapses in females than in males (***p = 0.0001, t(34) = 4.40). D, E, Density frequency distributions for all synapse-sized clusters of ERβ (D) and GPER1 (E) immunoreactivities colocalized with PSD-95 in the CA1 SR sample field (n = 12/group, females in diestrus). For ERβ-IR (D), there was a significant interaction between sex and immunolabeling density (p = 0.0005, F(21,462) = 2.4) because of a slightly greater proportion of synapses with low-density ERβ-IR (density units 83–88) in males than in females. There was no effect of sex on the numbers of densely ERβ-IR synapses (density units, ≥90; p = 0.12; t(22) = 1.6). For GPER1-IR, there were no group differences (GPER1: p = 0.89; F(20,440) = 0.33). F, Deconvolved epifluorescence images shows that ERβ and GPER1 (red) are both localized to synapse-sized puncta in CA1 SR and that some of those are colocalized with PSD-95 (green; doubles appear yellow). Scale bar, 2 μm.
    3d Montage, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+montage/pmc06136152-391-13-17?v=Oxford+Instruments
    Average 99 stars, based on 1 article reviews
    3d montage - by Bioz Stars, 2026-08
    99/100 stars
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    ERα is present at higher concentrations in female than in male CA1 synapses. A, Top left, Deconvolved two-photon microscopic images of immunofluorescent labeling were used to construct a 3D montage of the CA1 sample field (shown); one can see that a subpopulation of PSD-95-IR contacts (green) also contains ERα immunoreactivity (double labeling appears yellow). Scale bar, 2 μm. Top right, Image of a single double-labeled PSD shows the spatial relationship of areas occupied by PSD-95 (green) and ERα (red) immunoreactivities and the extent of overlap (merge, yellow). Scale bar, 0.1 μm. Bottom, Image shows the montage from the same z-stack illustrated in the top left but with the top of that panel rotated away from the viewer to show double labeling of the same puncta (arrows) from a different 3D viewpoint. B, The density frequency distribution for ERα-IR (colocalized with PSD-95) shows a greater rightward skew in females relative to males (p < 0.0001, F(19,646) = 17.28; males, n = 12; female, n = 24). C, Bar graph shows the percentage of double-labeled synapses with high concentrations of ERα immunolabeling (density units of ≥90) normalized to the mean male value shows that there were far more dense ERα-IR synapses in females than in males (***p = 0.0001, t(34) = 4.40). D, E, Density frequency distributions for all synapse-sized clusters of ERβ (D) and GPER1 (E) immunoreactivities colocalized with PSD-95 in the CA1 SR sample field (n = 12/group, females in diestrus). For ERβ-IR (D), there was a significant interaction between sex and immunolabeling density (p = 0.0005, F(21,462) = 2.4) because of a slightly greater proportion of synapses with low-density ERβ-IR (density units 83–88) in males than in females. There was no effect of sex on the numbers of densely ERβ-IR synapses (density units, ≥90; p = 0.12; t(22) = 1.6). For GPER1-IR, there were no group differences (GPER1: p = 0.89; F(20,440) = 0.33). F, Deconvolved epifluorescence images shows that ERβ and GPER1 (red) are both localized to synapse-sized puncta in CA1 SR and that some of those are colocalized with PSD-95 (green; doubles appear yellow). Scale bar, 2 μm.

    Journal: The Journal of Neuroscience

    Article Title: Memory-Related Synaptic Plasticity Is Sexually Dimorphic in Rodent Hippocampus

    doi: 10.1523/JNEUROSCI.0801-18.2018

    Figure Lengend Snippet: ERα is present at higher concentrations in female than in male CA1 synapses. A, Top left, Deconvolved two-photon microscopic images of immunofluorescent labeling were used to construct a 3D montage of the CA1 sample field (shown); one can see that a subpopulation of PSD-95-IR contacts (green) also contains ERα immunoreactivity (double labeling appears yellow). Scale bar, 2 μm. Top right, Image of a single double-labeled PSD shows the spatial relationship of areas occupied by PSD-95 (green) and ERα (red) immunoreactivities and the extent of overlap (merge, yellow). Scale bar, 0.1 μm. Bottom, Image shows the montage from the same z-stack illustrated in the top left but with the top of that panel rotated away from the viewer to show double labeling of the same puncta (arrows) from a different 3D viewpoint. B, The density frequency distribution for ERα-IR (colocalized with PSD-95) shows a greater rightward skew in females relative to males (p < 0.0001, F(19,646) = 17.28; males, n = 12; female, n = 24). C, Bar graph shows the percentage of double-labeled synapses with high concentrations of ERα immunolabeling (density units of ≥90) normalized to the mean male value shows that there were far more dense ERα-IR synapses in females than in males (***p = 0.0001, t(34) = 4.40). D, E, Density frequency distributions for all synapse-sized clusters of ERβ (D) and GPER1 (E) immunoreactivities colocalized with PSD-95 in the CA1 SR sample field (n = 12/group, females in diestrus). For ERβ-IR (D), there was a significant interaction between sex and immunolabeling density (p = 0.0005, F(21,462) = 2.4) because of a slightly greater proportion of synapses with low-density ERβ-IR (density units 83–88) in males than in females. There was no effect of sex on the numbers of densely ERβ-IR synapses (density units, ≥90; p = 0.12; t(22) = 1.6). For GPER1-IR, there were no group differences (GPER1: p = 0.89; F(20,440) = 0.33). F, Deconvolved epifluorescence images shows that ERβ and GPER1 (red) are both localized to synapse-sized puncta in CA1 SR and that some of those are colocalized with PSD-95 (green; doubles appear yellow). Scale bar, 2 μm.

    Article Snippet: Stacks were deconvolved using AutoQuant version 2.2.1 (Media Cybernetics) and constructed into a 3D montage using Imaris (Bitplane; Lauterborn et al., 2016 ).

    Techniques: Labeling, Construct, Immunolabeling